Genetic Analysis of Candida glabrata from Candiduric Patients Using Microsatellite Length Polymorphism, Antifungal Susceptibility, and Enzymatic Profiles

Background: Candida glabrata is the second agent of candiduria with increased resistance to antifungals. Microsatellite length polymorphism (MLP) one genotyping techniques used in epidemiological investigation improve clinical management. Objectives: We aimed detect different genotypes C. isolates using six microsatellite markers and MLP technique. Moreover, our genotypes' association other countries' was illustrated a minimum spanning tree. investigated vitro antifungal susceptibility enzymatic activity profiles isolates. Methods: Six were amplified multiplex-PCR for 22 strains isolated from urine pediatric patients admitted Abuzar Children's Hospital Ahvaz, Iran. The PCR products presented fragment analysis, size alleles determined. Antifungal tests extracellular enzyme activities also performed. Results: Nineteen multilocus detected so that 22.7% had identical genotypes. wild-type amphotericin B (0.0625 - 2 µg/mL), itraconazole (0.125 voriconazole (0.0078 0.00625 µg/mL). All sensitive fluconazole at inhibitory concentration (MIC) range (0.0312 16 μg/mL), three them resistant caspofungin (MIC ≥ 0.5 μg/mL). 72.7 68.2% no phospholipase esterase activities. highest potency obtained hemolysin proteinase enzymes. A high genetic diversity (19 isolates) existed among urinary Based on tree, two clusters related previous study Iran, third cluster entirely connected Chinese Conclusions: Most non-wild type posaconazole but rarely Hemolysin secreted as main virulence factors